Tissue Biorepository Core PDF *MNA User Authentication Required

Co-Scientific Directors

Chadrick E. Denlinger, MD
Assistant Professor, Department of Surgery

Richard R. Drake, PhD
Professor, Department of Cell and Molecular Pharmacology

Operations Manager
Kiki Gibbs
Phone: 843-876-2345
Fax: 843-876-2346
E-mail: gibbski@musc.edu
Location: BEB 421

Description
The Hollings Cancer Center Biorepository & Research Pathology Services Shared Resource provides investigators with a centralized infrastructure that promotes biomedical research involving the use and study of human biospecimens. The shared resource is comprised of four integrated components: Biospecimens and data bank, Laser Capture Microdissection, Tissue Microarray, and Research Pathology Services. These components, along with extensive staff expertise, offer a comprehensive means by which researchers can utilize valuable human biospecimens and cutting edge technology to support basic, translational and clinical research.

Resources
To inquire about or access Biorepository and Research Pathology Services, please contact the operations manager (contact information listed above).

Prior to services being performed, the requestor must submit an Order Form.  Upon receipt, the operations manager will contact the requestor, review the request, request additional information if needed, and provide a pricing proposal.  Please note: all requests for specimens undergo committee review for approval.

Useful Links:

• Order Form
• Patient Consent Form
  - Leukemia Requisition
  - Lymphoma Requisition
  - Myeloma Requisition 
• Fee Schedule
• Flowsheet to Determine Type of IRB Application
  - This tool assists investigators in determining what IRB review category is appropriate for their research.

Services 

• Biospecimen and Data Bank
  • Collecting, processing, and banking of tissue, saliva, urine, blood, plasma, serum, and other tissue derivatives; including those for protocol driven studies
  • Retrieval of banked specimens linked to clinicopathologic data, while maintaining patient confidentiality, for research use
  • Quality control of collected tissue by the Tissue Biorepository & Research Pathology Services Director, a trained pathologist: verification of diseased state and assessment of tumor purity, etc.
  • Quality control of DNA/RNA/protein isolated from collected tissue using the Agilent Bioanalyzer
• Laser Capture Microdissection
  • Identification, localization, and microdissection of targeted cell populations (from human and animal tissue sources)
  • Extraction of DNA/RNA/protein from microdissected samples.
  • Quality analysis and quality control of isolated nucleic acid using Agilent Bioanalyzer
• Tissue Microarray
  • Create custom and standard TMAs
  • Consultation and technical support in the construction and analyses of TMA
• Research Pathology Services
  • Macrodissection of tissue prior to isolation of DNA/RNA/protein to increase tumor purity
  • Immunohistochemistry and In-situ hybridization
  • Quantitative image analysis on conventional and TMA sections, including tissue scoring, Ki-67 labeling index, microvascular density counting, and tissue microarray scoring, etc.
• Bio-molecular Assessment
  • Cellular DNA, RNA and protein prepared by the Tissue Repository from banked specimens or any other biomolecules submitted by investigators can be qualitatively assessed by Agilent Bioanalyzer, prior to use for downstream applications such as microarray and/or qRT-PCR analysis

Publication Acknowledgement
If you publish a manuscript including research supported by the Biorepository and Research Pathology Services, the HCC recommends the following text to be placed in the acknowledgement section:
"Supported in part by the Biorepository & Research Pathology Services Shared Resource, Hollings Cancer Center, Medical University of South Carolina."

If you have any questions please e-mail the Biorepository & Research Pathology Services Shared Resource.