|Biorepository & Tissue Analysis Shared Resource|
Chadrick E. Denlinger, MD
Assistant Professor, Department of Surgery
Richard R. Drake, PhD
Professor, Department of Cell and Molecular Pharmacology
Location: BEB 421
The Hollings Cancer Center Biorepository & Research Pathology Services Shared Resource provides investigators with a centralized infrastructure that promotes biomedical research involving the use and study of human biospecimens. The shared resource is comprised of four integrated components: Biospecimens and data bank, Laser Capture Microdissection, Tissue Microarray, and Research Pathology Services. These components, along with extensive staff expertise, offer a comprehensive means by which researchers can utilize valuable human biospecimens and cutting edge technology to support basic, translational and clinical research.
To inquire about or access Biorepository and Research Pathology Services, please contact the operations manager (contact information listed above).
Prior to services being performed, the requestor must submit an Order Form. Upon receipt, the operations manager will contact the requestor, review the request, request additional information if needed, and provide a pricing proposal. Please note: all requests for specimens undergo committee review for approval.
- Biospecimen and Data Bank
- Collecting, processing, and banking of tissue, saliva, urine, blood, plasma, serum, and other tissue derivatives; including those for protocol driven studies
- Retrieval of banked specimens linked to clinicopathologic data, while maintaining patient confidentiality, for research use
- Quality control of collected tissue by the Tissue Biorepository & Research Pathology Services Director, a trained pathologist: verification of diseased state and assessment of tumor purity, etc.
- Quality control of DNA/RNA/protein isolated from collected tissue using the Agilent Bioanalyzer
- Laser Capture Microdissection
- Identification, localization, and microdissection of targeted cell populations (from human and animal tissue sources)
- Extraction of DNA/RNA/protein from microdissected samples.
- Quality analysis and quality control of isolated nucleic acid using Agilent Bioanalyzer
- Tissue Microarray
- Create custom and standard TMAs
- Consultation and technical support in the construction and analyses of TMA
- Research Pathology Services
- Macrodissection of tissue prior to isolation of DNA/RNA/protein to increase tumor purity
- Immunohistochemistry and In-situ hybridization
- Quantitative image analysis on conventional and TMA sections, including tissue scoring, Ki-67 labeling index, microvascular density counting, and tissue microarray scoring, etc.
- Bio-molecular Assessment
- Cellular DNA, RNA and protein prepared by the Tissue Repository from banked specimens or any other biomolecules submitted by investigators can be qualitatively assessed by Agilent Bioanalyzer, prior to use for downstream applications such as microarray and/or qRT-PCR analysis
If you publish a manuscript including research supported by the Biorepository and Research Pathology Services, the HCC recommends the following text to be placed in the acknowledgement section:
"Supported in part by the Biorepository & Research Pathology Services Shared Resource, Hollings Cancer Center, Medical University of South Carolina."
If you have any questions please e-mail the Biorepository & Research Pathology Services Shared Resource.