Flow Cytometry and Cell Sorting Unit
Amanda C. LaRue, PhD
Associate Professor, Pathology & Laboratory Medicine
Name: Adam Soloff, PhD
Research Assistant Professor, Microbiology & Immunology
Name: Gordon Wiegand
Location: 324 Hollings Cancer Center
- User-Based (Unassisted) Flow Cytometric Analysis: Equipment is available 24/7 for trained investigators. During regular working hours, operators are available to assist with setup, running samples, and trouble-shooting.
- Assisted Flow Cytometric Analysis: An operator is available for the FACSCalibur, LSRFortessa and FACSVerse during regular working hours to provide general assistance as well as standard assays including cell death, proliferation, cell cycle, Ca++ flux, etc.
- Cell Sorting: The MoFlo cell sorter is the instrument of choice for HCC members interested in sorting rare events or side-population cells as a means of isolating tissue stem cells and cancer stem-like cells. With the addition of an Aerosol Management System, the FACSAria IIu cell sorter is used for sorting populations with biohazard considerations. Due to complexity, all cell sorters are operated only by Flow Cytometry and Cell Sorting Unit staff.
- Rules and Regulations
- Analysis Software
- Educational Resources
- A Guide to Sample Preparation for Cell Sorting
If you publish a manuscript including research supported by the Flow Cytometry & Cell Sorting Shared Resource, the HCC recommends the following text to be placed in the acknowledgement section: "Supported in part by the Cell Evaluation & Therapy Shared Resource, Hollings Cancer Center, Medical University of South Carolina (P30 CA138313)."Comments