Lipidomics Shared Resource
Besim Ogretmen, PhD
Professor of Biochemistry & Molecular Biology
Alicja Bielawska, PhD
Research Professor of Biochemistry & Molecular Biology
The mission of the Lipidomics Shared Resource is to provide advanced methodology, state-of-the-art facilities, and expertise for both synthesis and analysis of bioactive lipids in a cost-effective manner for the Hollings Cancer Center. The Lipidomics Shared Resource represents a unique scientific service for the measurement of bioactive lipid molecules with a particular focus on their role in cancers. The Lipidomics Shared Resource is organized into two units – the Lipid Synthesis Unit and the Lipid Analysis Unit.
The purposes of the Lipidomics Shared Resource are to:
- Provide synthetic lipids, analogs, and inhibitors of lipid metabolism with an emphasis on sphingolipids.
- Provide qualitative and quantitative analysis of lipid composition from biological materials with a current listing of more than 300 distinct molecular species.
- Improve and develop new techniques for extended lipid analyses by providing a database of the lipid composition of normal and transformed cells and organelles that will permit a lipidomics approach to analysis of their cellular metabolism.
- Develop cutting-edge synthetic molecular tools to study the role of bioactive lipids as new potential anticancer agents.
- Develop lead compounds for translational studies. The Lipidomics Shared Resource’s library of synthetic lipids, derivatives, and related molecules currently exceeds 600 compounds, several of which are emerging as important inhibitors of enzymes of sphingolipid metabolism and being tested in clinical trials.
- Educate and assist HCC investigators in designing and conducting experimental approaches aimed at the study of bioactive lipids, their metabolism, quantitation, and function.
Equipment and Instrumentation
The Lipidomics Shared Resource has five functional chromatography/MS units that all use dual ionization modes: electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI).
- Four ThermoFisher TSQ Quantum triple-stage quadrupole mass spectrometers with super high pressure HPLC systems.
- A SCIEX 2000 Q-Trap triple quadrupole/ion trap combination instrument with outstanding sensitivity for improved detection limit and mass resolution connected to supercritical fluid chromatograph that allows separation and analysis of molecular species of GluCer and GalCer.
- The Lipidomics Shared Resource also has the following instrumentation for lipid sample preparation and MS analysis: Atlas nitrogen generator for providing a steady supply of high purity nitrogen for optimal performance for both MS instruments, Savant speed-vacuum evaporator, two nitrogen evaporators, Beckman centrifuge, and Shimadzu UV-1601 spectrophotometer.
The Lipidomics Shared Resource provides expertise in: sphingolipid chemistry and biology; qualitative and quantitative analysis of lipid components from biological samples (cells, tissue, biological fluids); determination of protein and total phospholipid levels in biological samples; homogenous sphingolipid standards; molecular tools to study sphingolipid metabolism; diversified synthetic sphingolipids and analogs for cellular, in vitro and in vivo studies. It also assists investigators in experimental design, selection of lipid of interest, and interpretation of analytical data.
If you publish a manuscript including research supported by the Lipidomics Shared Resource, the HCC recommends the following text to be placed in the acknowledgement section: "Supported in part by the Lipidomics Shared Resource, Hollings Cancer Center, Medical University of South Carolina (P30 CA138313)."