MUSC Hollings Cancer Center

Lipidomics - Analysis Unit

Manager & Lead Scientist
Jacek Bielawski, PhD
Research Professor, Biochemistry & Molecular Biology
843-792-1529
Contact: bielawsj@musc.edu
Location: 505C Children's Research Institute (CRI)

Personnel
Barbara Rembiesa, BS
843-792-7726

Jason Pierce, MS
843-792-7726

Aiping Bai, PhD
843-792-7726

Silva Terzieva, PhD
843-792-7726

Services

  • Qualitative (compound identification) and quantitative LC-MS/MS analysis of the key lipids from different biological materials (cells, tissues, serum, blood) determining their basal levels and changes in response to exogenous agents
  • Analyses of ~350 different sphingolipid and DAG components at a basic metabolomics level (please click here to view Doc. 2C1)
  • Methodology for the study of sphingolipid metabolism, thus eliminating the use of radioactive precursors (cell labeling with 17C sphingolipids and LC-MS/MS combined approach for analysis)
  • Methods for the determination of enzymatic activities at the cellular level (using 17C sphingolipids: e.g., 17C-Sph and LC-MS/MS combined approach for SK activity)
  • Determination of enzymatic activities of lipid metabolizing enzymes in vitro and ex vivo

The Lipidomics Analysis Unit has ~1200ft2 of dedicated laboratory and office space comprised of the following equipment:

  • Four (4) chromatography/mass spectrometer instruments with either ultra-high pressure HPLC systems or a supercritical fluid chromatograph (SFC) instrument
    These instruments are capable of dual ionization mode, electrospray ionization (ESI) and/or atmospheric pressure chemical ionization (APCI)
  • Nitrogen generator
  • Savant speed-vacuum evaporator 
  • Two (2) nitrogen evaporators 
  • Beckman centrifuge
  • Shimadzu UV-1601 spectrophotometer
  • Microbalance Metler Toledo

Lipidomics Analysis Unit Request Form

Analytical Units & Pricing

View all available analytical units and corresponding service prices.

Reference Citation 

The following text on lipid analyses by HPLC-MS/MS must be placed under the Methods Section of manuscripts:

“Sphingolipid levels were measured by high-performance liquid chromatography mass spectrometry (LC-MS/MS) methodology as previously described.
Bielawski, J., Pierce J.S., Snider J., Rembiesa B., Szulc Z.M., Bielawska A. (2009) Chapter 22 Comprehensive Quantitative Analysis of Bioactive Sphingolipids by High-Performance Liquid Chromatography–Tandem Mass Spectrometry, In D. Armstrong (Ed.), Lipidomics: Vol1: Methods and Protocols (pp. 443-467) New York, NY: Humana Press, a part of Springer Science+Business Media, LLC (Methods in Molecular Biology, Vol.579, ISBN: 978-1-60761-321-3)”

Data Presentation

Analytical results should be expressed as one of the following:

  • Lipid level (pmol) / Protein level (mg)
  • Lipid level (pmol) / Phospholipid level determined from the Bligh & Dyer lipid extract (nmol)
    Bligh, E .G., Dyer, W.J., (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911-917
  • Lipid level (pmol) / Volume (µL) reflecting in molar concentration [M]
  • Lipid level (pmol) / Tissue weight (mg)

Direct publications on the method development

 
 
 

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