Director:
Professor of Cell and Molecular Pharmacology
Contact Information:
Name: Daniel R. Knapp, Ph.D.
Phone: 843-792-5830
E-Mail: knappdr@musc.edu
Location: 305 Children's Research Institute
Description:
The aim of the Proteomics Core is to provide expertise, facilities, and services to facilitate application of proteomic analysis approaches to cancer research.
Services:
The Cancer Proteomics Core offers mass spectrometry services and identification of proteins separated by gel electrophoresis. Services include automated in-gel digestion, MALDI-TOF-TOF analysis of the resulting fragment peptides, and data interpretation using Mascot® software.
The Cancer Proteomics Core also offers a wide range of gel proteomics services including:
- 1 dimensional (1D) and 2-dimensional (2D) gel electrophoresis (PAGE and IPG/PAGE)
- 1D Isoelectric focusing
- Differential Gel Electrophoresis (DIGE)
- Native/ Blue Native Gels
- 2D Blue Native/PAGE
- 2D BN non-reducing/Native Reducing gels
- 2D westerns
- Detection of Phosphorylated and Glycated proteins
Equipment:
The mass spectrometry facility major equipment includes:
- Applied Biosystems 4700 Proteomics Analyzer [time of flight (TOF)-TOF tandem MS]
- Applied Biosystems QSTAR (Quadrupole-TOF tandem MS)
- Finnigan LCQ (Ion trap MS)
- 2 Finnigan LTQ (Ion trap MS)
- Applied Biosystems Voyager DE STR Biospectrometry Workstation (MALDI-TOF MS)
- Applied Biosystems Voyager DE Biospectrometry Workstation (MALDI-TOF MS)
- Applied Biosystems Mariner Biospectrometry Workstation (ESI-TOF MS)
- Packard Multiprobe II HT EX Liquid Handling System (in gel digestion robot)
The gel proteomics facility major equipment includes:
- GE Healthcare /Amersham IPGphore IPG isoelectric focusing system (12 strip)
- Ettan DaltSix Large Format (26 cm) 6 gel electrophoresis chamber
- BioRad Dodeca 11 cm Format 12 gel electrophoresis chamber
- AlphaInnotech 5500 (12 bit CCD camera) and 8900 (16 bit CCD camera) imagers for chemiluminescence, UV/Vis and fluorescence imaging(SyproRed/TexesRed/DeepPurple/ SybrGreen/GFP/SybrGold/YFP/Cy2/Cy3/Cy5)
- Typhoon imager with 3 laser excitation sources
